Since the last revision of the book, the methods used to study plant embryology have changed radically. Powerful modern biological techniques are now being applied to understand the developmental aspects and genetic and molecular bases of embryological processes. It has become possible to generate tissue specific mutants by T-DNA insertional mutagenesis, use of green fluorescent protein probes for live imaging of growing cells and tissues and to analyze gene expression in few-celled structures, such as early stages of embryo, and constituent cells of the male and female gametophytes.
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